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位置: 首页 > 磷酸化抗体 > DDR1 (Phospho-Tyr513) Antibody

DDR1 (Phospho-Tyr513) Antibody#11770

DDR1 (Phospho-Tyr513) Antibody
DDR1 (Phospho-Tyr513) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称DDR1 (Phospho-Tyr513) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu

特异性The antibody detects endogenous levels of DDR1 only when phosphorylated at tyrosine 513.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 513 (K-K-Y(p)-V-R) derived from Human DDR1 .

基因/蛋白名称DDR1

修饰Phospho

别名CAK; RTK6; TRKE; EDDR1;

数据库入口号Swiss-Prot#: Q08345;
NCBI Gene#: 780;
NCBI Protein#: NP_001189450.1.

UniprotQ08345

实际分子量110kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from JK cells (Lane 2), using DDR1 (Phospho-Tyr513) Antibody #11770. The lane on the left is treated with antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using DDR1 (Phospho-Tyr513) antibody #11770 (left)or the same antibody preincubated with blocking peptide (right).
背景

Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene is a RTK that is widely expressed in normal and transformed epithelial cells and is activated by various types of collagen. This protein belongs to a subfamily of tyrosine kinase receptors with a homology region to the Dictyostelium discoideum protein discoidin I in their extracellular domain. Its autophosphorylation is achieved by all collagens so far tested (type I to type VI).

di Marco E., J. Biol. Chem. 268:24290-24295(1993).
Johnson J.D., Proc. Natl. Acad. Sci. U.S.A. 90:5677-5681(1993).
Laval S., Cell Growth Differ. 5:1173-1183(1994).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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