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位置: 首页 > 磷酸化抗体 > CDC25C (Phospho-Ser198) Antibody

CDC25C (Phospho-Ser198) Antibody#11790

CDC25C (Phospho-Ser198) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称CDC25C (Phospho-Ser198) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用IHC

种属反应性Hu

特异性The antibody detects endogenous levels of CDC25C only when phosphorylated at serine 198.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 198(E-F-S(p)-L-K) derived from Human CDC25C.

基因/蛋白名称CDC25C

修饰Phospho

别名CDC25M1; MPI1B; MPIP3; EC 3.1.3.48;

数据库入口号Swiss-Prot#: P30307;
NCBI Gene#: 995;
NCBI Protein#: NP_001274511.1.

UniprotP30307

实际分子量53kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Immunohistochemistry: 1:50~1:100

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using CDC25C (Phospho-Ser198) antibody #11790 (left)or the same antibody preincubated with blocking peptide (right).
背景

cdc25C is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.

Sadhu K., Proc. Natl. Acad. Sci. U.S.A. 87:5139-5143(1990).
Bureik M., Int. J. Oncol. 17:1251-1258(2000).
Wegener S., Eur. J. Cell Biol. 79:810-815(2000).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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