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位置: 首页 > 磷酸化抗体 > MAPKAPK2 (Phospho-Ser272) Antibody

MAPKAPK2 (Phospho-Ser272) Antibody#11806

MAPKAPK2 (Phospho-Ser272) Antibody
MAPKAPK2 (Phospho-Ser272) Antibody
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产品总价:
产品详情

产品名称MAPKAPK2 (Phospho-Ser272) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous levels of MAPKAPK2 only when phosphorylated at serine 272.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 272(A-I-S(p)-P-G) derived from Human MAPKAPK2.

基因/蛋白名称MAPKAPK2

修饰Phospho

别名MAPK2; MAPKAPK2; RPS6KC1;

数据库入口号Swiss-Prot#: P49137;
NCBI Gene#: 9261;
NCBI Protein#: NP_116584.2.

UniprotP49137

实际分子量45kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from RAW264.7 cells treated with UV using MAPKAPK2 (Phospho-Ser272) Antibody #11806.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using MAPKAPK2 (Phospho-Ser272) antibody #11806 (left)or the same antibody preincubated with blocking peptide (right).
背景

This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.

Zu Y.-L., Biochem. Biophys. Res. Commun. 200-1118-1124(1994).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Stokoe D., Biochem. J. 296:843-849(1993).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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