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位置: 首页 > 磷酸化抗体 > MEF2C (Phospho-Ser396) Antibody

MEF2C (Phospho-Ser396) Antibody#11808

MEF2C (Phospho-Ser396) Antibody
MEF2C (Phospho-Ser396) Antibody
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产品总价:
产品详情

产品名称MEF2C (Phospho-Ser396) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms

特异性The antibody detects endogenous levels of MEF2C only when phosphorylated at serine 396.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 396(P-V-S(p)-P-P) derived from Human MEF2C.

基因/蛋白名称MEF2C

修饰Phospho

别名Myocyte enhancer factor 2C; Myocyte-specific enhancer factor 2C; Similar to MADS box transcription enhancer factor 2 polypeptide C;

数据库入口号Swiss-Prot#: Q06413;
NCBI Gene#: 4208;
NCBI Protein#: NP_001180279.1.

UniprotQ06413

实际分子量51kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from cos-7 cells (Lane 2), using MEF2C (Phospho-Ser396) Antibody #11808. The lane on the left is treated with antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using MEF2C (Phospho-Ser396) antibody #11808 (left)or the same antibody preincubated with blocking peptide (right).
背景

MEF2C transcription factor of the MADS family which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. May be involved in myogenesis, neurogenesis and in the development of cortical architecture. Three splice-variant isoforms have been described.

Leifer D., Proc. Natl. Acad. Sci. U.S.A. 90:1546-1550(1993).
McDermott J.C., Mol. Cell. Biol. 13:2564-2577(1993).
Wang A.H., Mol. Cell. Biol. 19:7816-7827(1999).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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