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位置: 首页 > 磷酸化抗体 > MuSK (Phospho-Tyr755) Antibody

MuSK (Phospho-Tyr755) Antibody#11837

MuSK (Phospho-Tyr755) Antibody
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产品总价:
产品详情

产品名称MuSK (Phospho-Tyr755) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous levels of MuSK only when phosphorylated at tyrosine 755.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 755(A-D-Y(p)-Y-K) derived from Human MuSK .

基因/蛋白名称MuSK

修饰Phospho

别名MUSK; Muscle; skeletal;

数据库入口号Swiss-Prot#: O15146;
NCBI Gene#: 4593;
NCBI Protein#: NP_005583.1.

UniprotO15146

实际分子量97kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000

Western blot analysis of extracts from JK cells (Lane 2), using MuSK (Phospho-Tyr755) Antibody #11837. The lane on the left is treated with antigen-specific peptide.
背景

Receptor tyrosine kinase which plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between the motor neuron and the skeletal muscle. Recruitment of AGRIN by LRP4 to the MUSK signaling complex induces phosphorylation and activation of MUSK, the kinase of the complex. The activation of MUSK in myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in subsynaptic nuclei, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors (AChR) in the postsynaptic membrane.

Valenzuela D.M., Neuron 15:573-584(1995).
Humphray S.J., Nature 429:369-374(2004).
Bergamin E., Mol. Cell 39:100-109(2010).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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