产品详情
产品名称AIF Rabbit mAb
克隆号SZ05-01
来源种属Recombinant Rabbit
克隆性Monoclonal antibody
纯化ProA affinity purified
应用WB, ICC/IF, IHC
种属反应性Human;Mouse;Rat
免疫原描述recombinant protein
标记Unconjugated
别名AIFM1 antibody
AIFM1_HUMAN antibody
Apoptosis inducing factor 1, mitochondrial antibody
Apoptosis inducing factor antibody
Apoptosis inducing factor, mitochondrion associated, 1 antibody
Apoptosis-inducing factor 1 antibody
CMTX4 antibody
COWCK antibody
COXPD6 antibody
Harlequin antibody
Hq antibody
mAIF antibody
MGC111425 antibody
MGC5706 antibody
mitochondrial antibody
Neuropathy, axonal motor-sensory, with deafness and mental retardation antibody
neuropathy, axonal, motor-sensory with deafness and mental retardation (Cowchock syndrome) antibody
PDCD 8 antibody
PDCD8 antibody
Programmed cell death 8 (apoptosis inducing factor) antibody
Programmed cell death 8 antibody
Programmed cell death 8 isoform 1 antibody
Programmed cell death 8 isoform 2 antibody
Programmed cell death 8 isoform 3 antibody
Programmed cell death protein 8 antibody
Programmed cell death protein 8 mitochondrial antibody
Programmed cell death protein 8 mitochondrial precursor antibody
Programmed cell death protein 8 mitochondrial precursor antibody
Striatal apoptosis inducing factor antibody
数据库入口号Swiss-Prot#:O95831
Uniprot
O95831
计算分子量67 kDa
实际分子量67 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-AIF antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AIF antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-AIF antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-AIF antibody. Counter stained with hematoxylin.
ICC staining AIF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining AIF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
All lanes : AIF Rabbit mAb at 1/1k dilution
Lane 1 : SKOV-3 whole cell lysates
Lane 2 : Hela whole cell lysates
Lane 3 : 3T3 whole cell lysates
Lane 4 : C6 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: 7 seconds
All lanes : AIF Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : AIF knockdown HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
A key event in the apoptotic process is the opening of the mitochondrial permeability transition pore, an event that is regulated by Bcl-2 family proteins, resulting in the release of several proteins from the mitochondrial intermembrane space. Several of these proteins participate in apoptosis, including cytochrome c, procaspases 2, 3, and 9, and AIF (apoptosis-inducing factor). AIF has been shown to cause DNA fragmentation and chromatin condensation and to induce the release of cytochrome c and caspase-9 from mitochondria. Bcl-2 overexpression has been shown to prevent the release of AIF from mitochondria, but not to block its apoptogenic activity.
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