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位置: 首页 > 重组兔单抗 > ATPB Rabbit mAb

ATPB Rabbit mAb#49442

是否有货: Yes
产品详情

产品名称ATPB Rabbit mAb

克隆号JM10-90

来源种属Recombinant Rabbit

克隆性Monoclonal antibody

纯化ProA affinity purified

应用WB, ICC/IF, IHC

种属反应性Human;Mouse;Rat

免疫原描述recombinant protein

标记Unconjugated

别名ATP 5B antibody
ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody
ATP synthase subunit beta mitochondrial antibody
ATP synthase subunit beta, mitochondrial antibody
atp5b antibody
ATPB antibody
ATPB_HUMAN antibody
ATPMB antibody
ATPSB antibody
Epididymis secretory protein Li 271 antibody
HEL-S-271 antibody
Mitochondrial ATP synthase beta subunit antibody
Mitochondrial ATP Synthase Subunit Beta antibody
Mitochondrial ATP synthetase beta subunit antibody

数据库入口号Swiss-Prot#:P06576

Uniprot P06576

计算分子量57 kDa

实际分子量53 kDa

配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

保存Store at -20˚C

应用详情
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
Western blot analysis of ATPB on different cells lysates using anti-ATPB antibody at 1/500 dilution. Positive control: Line1: Hela Line2: HepG2 Line3: 293T
Western blot analysis of ATPB on Zebrafish cells lysates using anti-ATPB antibody at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ATPB antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ATPB antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ATPB antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded huaman uterus tissue using anti-ATPB antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ATPB antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ATPB antibody. Counter stained with hematoxylin.
ICC staining ATPB in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ATPB in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ATPB in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
All lanes : ATPB Rabbit mAb at 1/1k dilution
Lane 1 : Rat spleen lysates whole cell lysates
Lane 2 : Mouse spleen lysates whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 53 kDa
Exposure time: 6 seconds
All lanes : ATPB Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : ATPB knockdown HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

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注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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