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位置: 首页 > 磷酸化抗体 > HER2(Phospho-Tyr877) Antibody

HER2(Phospho-Tyr877) Antibody#11075

HER2(Phospho-Tyr877) Antibody
HER2(Phospho-Tyr877) Antibody
HER2(Phospho-Tyr877) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称HER2(Phospho-Tyr877) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of HER2 only when phosphorylated at tyrosine 877.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 877 (T-E-Y(p)-H-A) derived from Human HER2.

基因/蛋白名称HER2

修饰Phospho

别名C-erbB-2; ErbB2;

数据库入口号Swiss-Prot: P04626
NCBI Protein: NP_001005862.1

UniprotP04626

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 185kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from A431 cells untreated or treated with EGF using HER2(Phospho-Tyr877) Antibody #11075.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using HER2(Phospho-Tyr877) Antibody #11075(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed MCF7 cells using HER2(Phospho-Tyr877) Antibody #11075.
背景
Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-a and amphiregulin.

Dittadi, R. et al. (2000) J. Natl. Cancer Inst. 92, 1443-1444.
Muthuswamy, S. K. et al. (1999) Mol. Cell. Biol. 19, 6845-6857.
Qian, X. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 1500-1504.

如果您使用该产品11075发表了文章,请通知我们,让我们可以引用您的文献。

M.Alicia Corte?s, Ariel E.Cariaga-Martinez, Mar??a V.T.Lobo et al,EGF promotes neuroendocrine-like differentiation of prostate cancer cells in the presence of LY294002 through increased ErbB2 expression independent of the phosphatidylinositol 3-kinase-AKT pathway, Carcinogenesis, vol.33 no.6 pp.1169篓C1177 (2012),
PMID: 22461520
et al,EGF promotes neuroendocrine-like differentiation of prostate cancer cells in the presence of LY294002 through increased ErbB2 expression independent of the phosphatidylinositol 3-kinase-AKT pathway. In Carcinogenesis on 2012 Jun by Javier Angulo, Pilar L贸pez-Ruiz, et al..PMID: 22461520, , (2012),
PMID: 22461520
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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