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位置: 首页 > 内参抗体 > β-actin Mouse Monoclonal Antibody

β-actin Mouse Monoclonal Antibody#38074

β-actin Mouse Monoclonal Antibody
β-actin Mouse Monoclonal Antibody
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产品总价:
产品详情

产品名称β-actin Mouse Monoclonal Antibody

来源种属Mouse

克隆性Monoclonal

克隆号5B7

纯化Affinity purification using immunogen.

应用WB IHC IF

种属反应性Hu Rt Ms Mk Dg Chk Hm Rb Pg Sh

特异性Antibody detects endogenous β-actin protein.

基因/蛋白名称β-actin

别名ACTB; BRWS1; PS1TP5BP1

数据库入口号Swiss-Prot#:P60709

UniprotP60709

实际分子量45kd

浓度1.0mg/ml

配方Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情
Western blotting: 1:5000~1:10000
Immunohistochemistry: 1:200
Western blot analysis of 1) 293T ,2) Mouse Kidney tissue, 3) Hela, 4) Rat Heart tissue, 5) Rat Brain tissue, using #38074 diluted at 1:5,000.
IHC staining of Human ovary tissue with β-Actin mouse mAb(5B7) diluted at 1:200.
背景
β-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells. For example, expression of β-actin in adipose tissue is very low and therefore β-actin should not be used as loading control for these tissues.

如果您使用该产品38074发表了文章,请通知我们,让我们可以引用您的文献。

et al,Gut-Flora-Dependent Metabolite Trimethylamine-N-Oxide Promotes Atherosclerosis-Associated Inflammation Responses by Indirect ROS Stimulation and Signaling Involving AMPK and SIRT1. In Nutrients on 2022 Aug 15 by Sa Zhou, Jiamin Xue, et al..PMID:36014845, , (2022),
PMID: 36014845
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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