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位置: 首页 > 磷酸化抗体 > CDC25B (Phospho-Ser323) Antibody

CDC25B (Phospho-Ser323) Antibody #12104

CDC25B (Phospho-Ser323) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称CDC25B (Phospho-Ser323) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous levels of CDC25B only when phosphorylated at serine 323.

免疫原类型peptide

免疫原描述Peptide sequence around phosphorylation site of serine 323 (S-P-S(p)-M-P) derived from Human CDC25B.

基因/蛋白名称CDC25B

修饰Phospho

别名CDC25HU2; CDC25M2; Dual specificity phosphatase Cdc25B; EC 3.1.3.48; M-phase inducer phosphatase 2; MPIP2

数据库入口号Swiss-Prot#:P30305;
NCBI Gene#:994

UniprotP30305

实际分子量62kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情
Western blotting: 1:500~1:3000
Western blot analysis of extracts from NIH/3T3 cells treated with PMA (125ng/ml, 30mins), using CDC25B (Phospho-Ser323) antibody #12104. The lane on the right is treated with the synthesized peptide.
背景
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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