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位置: 首页 > 磷酸化抗体 > ERK8 (Phospho-Thr175/Tyr177) Antibody

ERK8 (Phospho-Thr175/Tyr177) Antibody#12105

ERK8 (Phospho-Thr175/Tyr177) Antibody
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产品总价:
产品详情

产品名称ERK8 (Phospho-Thr175/Tyr177) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms

特异性The antibody detects endogenous levels of ERK8 only when phosphorylated at threonine 175 and tyrosine 177.

免疫原类型peptide

免疫原描述Peptide sequence around phosphorylation site of threonine 175 and tyrosine 177 (A-V-T(p)-E-Y(p)-V-A) derived from Human ERK8.

基因/蛋白名称ERK8

修饰Phospho

别名EC 2.7.11.24; Extracellular signal-regulated kinase 8

数据库入口号Swiss-Prot#:Q8TD08;
NCBI Gene#:225689

UniprotQ8TD08

实际分子量60kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情
Western blotting: 1:500~1:3000
Western blot analysis of extracts from Jurkat cells, using ERK8 (Phospho-Thr175+Tyr177) antibody #12105. The lane on the right is treated with the synthesized peptide.
背景
In vitro, phosphorylates MBP.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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