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位置: 首页 > 磷酸化抗体 > HBP1 (Phospho-Ser402) Antibody

HBP1 (Phospho-Ser402) Antibody#12107

HBP1 (Phospho-Ser402) Antibody
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产品总价:
产品详情

产品名称HBP1 (Phospho-Ser402) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms

特异性The antibody detects endogenous levels of HBP1 only when phosphorylated at serine 402.

免疫原类型peptide

免疫原描述Peptide sequence around phosphorylation site of serine 402 (C-G-S(p)-P-G) derived from Human HBP1.

基因/蛋白名称HBP1

修饰Phospho

别名HMG box containing protein 1

数据库入口号Swiss-Prot#:O60381;
NCBI Gene#:26959

UniprotO60381

实际分子量58kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情
Western blotting: 1:500~1:3000
Western blot analysis of extracts from A549 cells, treated with PMA (125ng/ml, 30mins), using HBP1 (Phospho-Ser402) antibody #12107. The lane on the right is treated with the synthesized peptide.
背景
Transcriptional repressor that binds to the promoter region of target genes. Plays a role in the regulation of the cell cycle and of the Wnt pathway. Binds preferentially to the sequence 5'-TTCATTCATTCA-3'. Binding to the H1F0 promoter is enhanced by interaction with RB1. Disrupts the interaction between DNA and TCF4.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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Email Address

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