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位置: 首页 > 磷酸化抗体 > Kir6.2 (Phospho-Thr224) Antibody

Kir6.2 (Phospho-Thr224) Antibody#12108

Kir6.2 (Phospho-Thr224) Antibody
Kir6.2 (Phospho-Thr224) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Kir6.2 (Phospho-Thr224) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IF

种属反应性Hu Ms

特异性The antibody detects endogenous levels of Kir6.2 only when phosphorylated at threonine 224.

免疫原类型peptide

免疫原描述Peptide sequence around phosphorylation site of threonine 224 (K-T-TP-S-P) derived from Human Kir6.2.

基因/蛋白名称Kir6.2

修饰Phospho

别名ATP-sensitive inward rectifier potassium channel 11; IKATP; IRK11; Inward rectifier K channel Kir6.2; KCNJ11; Potassium channel; inwardly rectifying; subfamily J; member 11

数据库入口号Swiss-Prot#:Q14654;
NCBI Gene#:3767

UniprotQ14654

实际分子量40kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Western blot analysis of extracts from HeLa cells, using Kir6.2 (Phospho-Thr224) antibody #12108. The lane on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HuvEc cells, using Kir6.2 (Phospho-Thr224) antibody #12108. The picture on the right is treated with the synthesized peptide.
背景
This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium By similarity. Subunit of ATP-sensitive potassium channels (KATP). Can form cardiac and smooth muscle-type KATP channels with ABCC9. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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