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位置: 首页 > 磷酸化抗体 > TOP2A (Phospho-Ser1106) Antibody

TOP2A (Phospho-Ser1106) Antibody#12110

TOP2A (Phospho-Ser1106) Antibody
TOP2A (Phospho-Ser1106) Antibody
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产品总价:
产品详情

产品名称TOP2A (Phospho-Ser1106) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms

特异性The antibody detects endogenous levels of TOP2A only when phosphorylated at serine 1106.

免疫原类型peptide

免疫原描述Peptide sequence around phosphorylation site of serine 1106 (E-E-S(p)-D-N) derived from Human TOP2A.

基因/蛋白名称TOP2A

修饰Phospho

别名alpha isozyme; DNA topoisomerase II; DNA topoisomerase II-alpha; EC 5.99.1.3; P11388-1; TOP-2; TOP2; TOP2A; TP2A

数据库入口号Swiss-Prot#:P11388;
NCBI Gene#:7153

UniprotP11388

实际分子量174kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Jurkat cells, treated with paclitaxel (1uM, 24hours), using TOP2A (Phospho-Ser1106) antibody #12110. The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using TOP2A (Phospho-Ser1106) antibody #12110. The picture on the right is treated with the synthesized peptide.
背景
Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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