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位置: 首页 > 磷酸化抗体 > Retinoic Acid Receptor α (Phospho-Ser77) Antibody

Retinoic Acid Receptor α (Phospho-Ser77) Antibody#12121

Retinoic Acid Receptor α (Phospho-Ser77) Antibody
Retinoic Acid Receptor α (Phospho-Ser77) Antibody
Retinoic Acid Receptor α (Phospho-Ser77) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Retinoic Acid Receptor α (Phospho-Ser77) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous levels of Retinoic Acid Receptor α only when phosphorylated at serine 77.

免疫原类型peptide

免疫原描述Peptide sequence around phosphorylation site of serine 77 (P-P-S(p)-P-P) derived from Human Retinoic Acid Receptor α.

基因/蛋白名称Retinoic Acid Receptor α

修饰Phospho

别名NR1B1; RAR-alpha; Retinoic acid receptor alpha; retinoic acid receptor; alpha; RRA

数据库入口号Swiss-Prot#:P10276;
NCBI Gene#:5914

UniprotP10276

实际分子量45kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Jurkat cells treated with PMA (125ng/ml, 30mins), using Retinoic Acid Receptor α(Phospho-Ser77) antibody #12121. The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from JK cells (Lane 2) and COS7 cells (Lane 3), using Retinoic Acid Receptor α (Phospho-Ser77) Antibody #12121. The lane on the left is treated with synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Retinoic Acid Receptor α (Phospho-Ser77) antibody #12121. The picture on the right is treated with the synthesized peptide.
背景
Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function By similarity. Regulates expression of target genes in a ligand-dependent manner by recruiting chromatin complexes containing KMT2E/MLL5. Mediates retinoic acid-induced granulopoiesis.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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