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位置: 首页 > 磷酸化抗体 > Cyclin D2 (Phospho-Thr280) Antibody

Cyclin D2 (Phospho-Thr280) Antibody#12122

Cyclin D2 (Phospho-Thr280) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Cyclin D2 (Phospho-Thr280) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous levels of Cyclin D2 only when phosphorylated at threonine 280.

免疫原类型peptide

免疫原描述Peptide sequence around phosphorylation site of threonine 280 (A-S-T(p)-P-T) derived from Human Cyclin D2.

基因/蛋白名称Cyclin D2

修饰Phospho

别名cyclin D2; G1/S-specific cyclin D2

数据库入口号Swiss-Prot#:P30279;
NCBI Gene#:894

UniprotP30279

实际分子量40kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情
Western blotting: 1:500~1:3000
Western blot analysis of extracts from COLO cells, treated with EGF (200ng/ml, 30mins), using Cyclin D2 (Phospho-Thr280) antibody #12122. The lane on the right is treated with the synthesized peptide.
背景
Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex By similarity.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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