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位置: 首页 > 磷酸化抗体 > hnRNP K (Phospho-Ser216) Antibody

hnRNP K (Phospho-Ser216) Antibody#12140

hnRNP K (Phospho-Ser216) Antibody
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产品总价:
产品详情

产品名称hnRNP K (Phospho-Ser216) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous levels of hnRNP K only when phosphorylated at serine 216.

免疫原类型peptide

免疫原描述Peptide sequence around phosphorylation site of serine 216 (S-E-S(p)-P-I) derived from Human hnRNP K.

基因/蛋白名称hnRNP K

修饰Phospho

别名Heterogeneous nuclear ribonucleoprotein K;hnRNP K;Transformation up-regulated nuclear protein; TUNP

数据库入口号Swiss-Prot#:P61978;
NCBI Gene#:3190

UniprotP61978

实际分子量55kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情
Western blotting: 1:500~1:3000
Western blot analysis of extracts from JurKat cells, using hnRNP K (Phospho-Ser216) antibody #12140. The lane on the right is treated with the synthesized peptide.
背景
One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction By similarity. As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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