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位置: 首页 > 磷酸化抗体 > MNK1 (Phospho-Thr255) Antibody

MNK1 (Phospho-Thr255) Antibody#12142

MNK1 (Phospho-Thr255) Antibody
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产品总价:
产品详情

产品名称MNK1 (Phospho-Thr255) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous levels of MNK1 only when phosphorylated at threonine 255.

免疫原类型peptide

免疫原描述Peptide sequence around phosphorylation site of threonine 255 (L-T-T(p)-P-C) derived from Human MNK1.

基因/蛋白名称MNK1

修饰Phospho

别名EC 2.7.11.1; kinase Mnk1; Map kinase interacting kinase; MAP kinase signal-integrating kinase 1; MAP kinase-interacting serine/threonine kinase 1; MKNK1

数据库入口号Swiss-Prot#:Q9BUB5;
NCBI Gene#:8569

UniprotQ9BUB5

实际分子量60kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情
Western blotting: 1:500~1:3000
Western blot analysis of extracts from HeLa cells, treated with Adriamycin (0.5ug/ml, 24hours), using MNK1 (Phospho-Thr255) antibody #12142. The lane on the right is treated with the synthesized peptide.
背景
May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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