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位置: 首页 > 磷酸化抗体 > Mst1 (Phospho-Thr183) Antibody

Mst1 (Phospho-Thr183) Antibody#12144

Mst1 (Phospho-Thr183) Antibody
Mst1 (Phospho-Thr183) Antibody
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产品总价:
产品详情

产品名称Mst1 (Phospho-Thr183) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms

特异性The antibody detects endogenous levels of Mst1 only when phosphorylated at threonine 183.

免疫原描述Peptide sequence around phosphorylation site of threonine 183 (R-N-T(p)-V-I) derived from Human Mst1.

基因/蛋白名称Mst1

修饰Phospho

别名EC 2.7.11.1; MST-1; Mammalian STE20-like protein kinase 1; STE20-like kinase MST1; STK4; Serine/threonine protein kinase 4; Serine/threonine protein kinase Krs-2; kinase MST1; serine/threonine kinase 4; STE20-like kinase MST1; Yeast Sps1/Ste20-related kin

数据库入口号Swiss-Prot#:Q13043;
NCBI Gene#:6789

UniprotQ13043

实际分子量60kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
 

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Mst1 (Phospho-Thr183) antibody #12144. The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from 293 cells, treated with H2O2 (100uM, 15mins), using Mst1 (Phospho-Thr183) antibody #12144. The lane on the right is treated with the synthesized peptide.
背景

Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation By similarity.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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