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位置: 首页 > 磷酸化抗体 > p16-INK4a (Phospho-Ser152) Antibody

p16-INK4a (Phospho-Ser152) Antibody#12148

p16-INK4a (Phospho-Ser152) Antibody
p16-INK4a (Phospho-Ser152) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称p16-INK4a (Phospho-Ser152) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu

特异性The antibody detects endogenous levels of p16-INK4a only when phosphorylated at serine 152.

免疫原类型peptide

免疫原描述Peptide sequence around phosphorylation site of serine 152 (G-P-S(p)-D-I) derived from Human p16-INK4a.

基因/蛋白名称p16-INK4a

修饰Phospho

别名CD2A1; CDK4I; CDKN2; CDKN2A; CDN2; Cyclin-dependent kinase 4 inhibitor A; cyclin-dependent kinase inhibitor 2A; MTS1; Multiple tumor suppressor 1; p14ARF; p16(INK4a); p16-INK4; P16INK4A

数据库入口号Swiss-Prot#:P42771;
NCBI Gene#:1029

UniprotP42771

实际分子量20kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from HeLa cells, treated with EPO (20U/ml, 15mins), using p16-INK4a (Phospho-Ser152) antibody #12148. The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using p16-INK4a (Phospho-Ser152) antibody #12148. The picture on the right is treated with the synthesized peptide.
背景
Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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