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位置: 首页 > 磷酸化抗体 > TAK1 (Phospho-Thr184) Antibody

TAK1 (Phospho-Thr184) Antibody#12153

TAK1 (Phospho-Thr184) Antibody
TAK1 (Phospho-Thr184) Antibody
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产品总价:
产品详情

产品名称TAK1 (Phospho-Thr184) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous levels of TAK1 only when phosphorylated at threonine 184.

免疫原类型peptide

免疫原描述Peptide sequence around phosphorylation site of threonine 184 (I-Q-T(p)-H-M) derived from Human TAK1.

基因/蛋白名称TAK1

修饰Phospho

别名EC 2.7.11.25; M3K7; MAP3K7; Mitogen-activated protein kinase kinase kinase 7; TGF-beta- activated kinase 1; Transforming growth factor-beta-activated kinase 1; kinase TAK1

数据库入口号Swiss-Prot#:O43318;
NCBI Gene#:6885

UniprotO43318

实际分子量65kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情
Western blotting: 1:500~1:3000
Western blot analysis of extracts from HepG2 cells, treated with TNF (20ng/ml, 5mins), using TAK1 (Phospho-Thr184) antibody #12153. The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from 293 cells (Lane 2), using TAK1 (Phospho-Thr184) Antibody #12153. The lane on the left is treated with synthesized peptide.
背景
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR). Ceramides are also able to activate MAP3K7/TAK1. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7.

如果您使用该产品12153发表了文章,请通知我们,让我们可以引用您的文献。

et al,PGC-1β suppresses saturated fatty acid-induced macrophage inflammation by inhibiting TAK1 activation. In IUBMB Life on 2016 Feb by Hongen Chen, Yan Liu, et al..PMID: 26748475 , , (2016),
PMID: 26748475
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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