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位置: 首页 > 磷酸化抗体 > UBF (Phospho-Ser484) Antibody

UBF (Phospho-Ser484) Antibody #12154

UBF (Phospho-Ser484) Antibody
UBF (Phospho-Ser484) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称UBF (Phospho-Ser484) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous levels of UBF only when phosphorylated at serine 484.

免疫原类型peptide

免疫原描述Peptide sequence around phosphorylation site of serine 484 (P-E-S(p)-P-K) derived from Human UBF.

基因/蛋白名称UBF

修饰Phospho

别名Autoantigen NOR-90; Nucleolar transcription factor 1; TCFUBF; UBF-1; UBF1; UBTF; Upstream binding factor 1

数据库入口号Swiss-Prot#:P17480;
NCBI Gene#:7343

UniprotP17480

实际分子量85kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from HeLa cells, treated with calyculinA (50ng/ml, 30mins), using UBF (Phospho-Ser484) antibody #12154. The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using UBF (Phospho-Ser484) antibody #12154. The picture on the right is treated with the synthesized peptide.
背景
Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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