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MLC2 (Phospho-Tyr118) Antibody#11589

MLC2 (Phospho-Tyr118) Antibody
MLC2 (Phospho-Tyr118) Antibody
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产品总价:
产品详情

产品名称MLC2 (Phospho-Tyr118) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IF

种属反应性Hu

特异性The antibody detects endogenous level of MLC2 only when phosphorylated at tyrosine 118.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 118 (A-D-Y(p)-V-R) derived from Human MLC2.

基因/蛋白名称MLC2

修饰Phospho

别名MLC2; MRLC1; MYRL2

数据库入口号Swiss-Prot#: P10916
NCBI Gene ID: 4633
NCBI mRNA#: NM_000432.3. .
NCBI Protein#: NP_000423.2.

UniprotP10916

实际分子量20kd

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C

应用详情

 Western blotting: 1:500~1:1000

 Immunofluorescence: 1:100~1:200

Immunofluorescence staining of methanol-fixed U87 cells using MLC2 (Phospho-Tyr118) Antibody #11589.
背景
 Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
 
1) Xia, Y. et al. c-Jun downregulation by HDAC3-dependent transcriptional repression promotes osmotic stress-induced cell apoptosis. Mol. Cell 25, 219–232 (2007).
2)Vander Heiden, M. G. et al. Evidence for an alternative glycolytic pathway in rapidly proliferating cells. Science 329, 1492–1499 (2010).
3) Fang, D. et al. Phosphorylation of beta-catenin by AKT promotes beta-catenin transcriptional activity. J. Biol. Chem. 282, 11221–11229 (2007).

如果您使用该产品11589发表了文章,请通知我们,让我们可以引用您的文献。

Zhimin Lu,Yuhui Jiang, Yugang Wang,et al. et al,PKM2 phosphorylates MLC2 and regulates cytokinesis of tumour cells, Nature Communications, 10.1038 (2014),
PMID: 25412762
et al,PKM2 phosphorylates MLC2 and regulates cytokinesis of tumour cells.In Nat Commun on 2014 Nov 21 by Yuhui Jiang , Yugang Wang et al..PMID: 25412762, , (2014),
PMID: 25412762
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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