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位置: 首页 > 磷酸化抗体 > MDM2(Phospho-Ser260) antibody

MDM2(Phospho-Ser260) antibody#12171

MDM2(Phospho-Ser260) antibody
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产品总价:
产品详情

产品名称MDM2(Phospho-Ser260) antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous level of MDM2 only when phosphorylated at serine 260.

免疫原类型Peptide

免疫原描述A phospho specific peptide corresponding to residues surrounding S260 of human MDM2.

基因/蛋白名称MDM2

修饰Phospho

别名HDMX; hdm2; ACTFS; MGC5370; MGC71221

数据库入口号Swiss-Prot#: Q00987
NCBI Gene ID: 4193

UniprotQ00987

实际分子量95kD;55KD(Calculated)

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情

Western blotting: 1:500 - 1:2000

Western blot analysis of extracts of 293 cell line, using phospho-MDM2-S260 antibody.
背景

MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 (1). Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 (2-4). Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 (3).

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注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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025-58868422

Email Address

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