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PKM2(Phospho-Ser37) Antibody#12859

PKM2(Phospho-Ser37) Antibody
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产品总价:
产品详情

产品名称PKM2(Phospho-Ser37) Antibody

来源种属Rabbit

克隆性Polyclonal

应用WB

种属反应性Hu Ms Rt

特异性Phospho-PKM2(S37) Antibody detects endogenous levels of PKM2 only when phosphorylated at S37

免疫原类型Peptide-KLH

免疫原描述A synthesized peptide derived from human PKM2(Phospho-Ser37)

别名CTHBP antibody Cytosolic thyroid hormone binding protein antibody Cytosolic thyroid hormone-binding protein antibody KPYM_HUMAN antibody MGC3932 antibody OIP 3 antibody OIP-3 antibody OIP3 antibody OPA interacting protein 3 antibody Opa-interacting protein 3 antibody p58 antibody PK muscle type antibody PK muscle type antibody PK2 antibody PK3 antibody PKM antibody PKM2 antibody pykm antibody Pyruvate kinase 2 3 antibody Pyruvate kinase 3 antibody Pyruvate kinase isozymes M1 M2 antibody Pyruvate kinase muscle antibody Pyruvate kinase muscle isozyme antibody pyruvate kinase PKM antibody Pyruvate kinase muscle 2 antibody TCB antibody THBP1 antibody Thyroid hormone binding protein 1 antibody Thyroid hormone binding protein cytosolic antibody Thyroid hormone-binding protein 1 antibody Tumor M2 PK antibody Tumor M2-PK antibody

数据库入口号Swiss-Prot#:P14618
NCBI Gene ID5315

计算分子量60

浓度1.0mg mL

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+) pH 7.4 150mM NaCl 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情
WB dilution:1:1000
Western blot analysis PKM2(Phospho-Ser37) using PMA treated NIH-3T3 whole cell lysates
产品描述
Pyruvate kinase, a glycolytic enzyme, catalyses the conversion of phosphoenolpyruvate to pyruvate. In mammals, the M1 isoform (PKM1) is expressed in most adult tissues (1). The M2 isoform (PKM2), an alternatively-spliced variant of M1, is expressed during embryonic development (1). Studies found that cancer cells exclusively express PKM2 (1-3). PKM2 is shown to be essential for aerobic glycolysis in tumors (Warburg effect) (1). When the M2 isoform is switched to the M1 isoform, aerobic glycolysis is reduced and oxidative phosphorylation is increased in cancer cells (1). These cells also show decreased tumorigenicity in mouse xenografts (1). Recent studies show that the oncogenic forms of FGFR1 directly phosphorylate Tyr105 of PKM2 and thereby inhibit the formation of active tetrameric PKM2 (4). A PKM2 mutant found in cancer cells, in which Tyr105 is replaced by phenylalanine, leads to reduced cell proliferation in hypoxia and tumor growth in xenografts in nude mice (4). These findings suggest that the phosphorylation at Tyr105 is a critical switch for the metabolism in cancer cells that promotes tumor growth (4).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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