Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Fibronectin

protein_function: Integrins alpha-1,beta-1, alpha-2,beta-1, alpha-10,beta-1 and alpha-11,beta-1 are receptors for collagen. Integrins alpha-1,beta-1 and alpha-2,beta-2 recognize the proline-hydroxylatedsequence G-F-P-G-E-R in collagen. Integrins alpha-2,beta-1, alpha-3,beta-1, alpha-4,beta-1, alpha-5,beta-1, alpha-8,beta-1, alpha-10,beta-1, alpha-11,beta-1 and alpha-V,beta-1 are receptors forfibronectin. Alpha-4,beta-1 recognizes one or more domains withinthe alternatively spliced CS-1 and CS-5 regions of fibronectin.Integrin alpha-5,beta-1 is a receptor for fibrinogen. Integrinalpha-1,beta-1, alpha-2,beta-1, alpha-6,beta-1 and alpha-7,beta-1are receptors for lamimin. Integrin alpha-4,beta-1 is a receptorfor VCAM1 and recognizes the sequence Q-I-D-S in VCAM1. Integrinalpha-9,beta-1 is a receptor for VCAM1, cytotactin andosteopontin. It recognizes the sequence A-E-I-D-G-I-E-L incytotactin. Integrin alpha-3,beta-1 is a receptor for epiligrin,thrombospondin and CSPG4. Integrin alpha-3,beta-1 provides adocking site for FAP (seprase) at invadopodia plasma membranes ina collagen-dependent manner and hence may participate in theadhesion, formation of invadopodia and matrix degradationprocesses, promoting cell invasion. Alpha-3,beta-1 may mediatewith LGALS3 the stimulation by CSPG4 of endothelial cellsmigration. Integrin alpha-V,beta-1 is a receptor for vitronectin.Beta-1 integrins recognize the sequence R-G-D in a wide array ofligands. When associated with alpha-7,beta-1 integrin, regulatescell adhesion and laminin matrix deposition. Involved in promotingendothelial cell motility and angiogenesis. Involved in osteoblastcompaction through the fibronectin fibrillogenesis cell-mediatedmatrix assembly process and the formation of mineralized bonenodules. May be involved in up-regulation of the activity ofkinases such as PKC via binding to KRT1. Together with KRT1 andGNB2L1, serves as a platform for SRC activation or inactivation.Plays a mechanistic adhesive role during telophase, required forthe successful completion of cytokinesis..
Fibronectin(FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides. These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris. Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer.