产品详情
产品名称Human PCSK9 ELISA Kit
特异性Human
交叉反应性There is no detectable cross-reactivity with other relevant proteins.
免疫原类型NSO,S153-Q692
别名Proprotein convertase subtilisin,kexin type 9; 3.4.21.-; Neural apoptosis-regulated convertase 1; NARC-1; Proprotein convertase 9; PC9; Subtilisin,kexin-like protease PC9; PCSK9; NARC1; PSEC0052;
数据库入口号Q8NBP7
细胞定位 Cytoplasm. Secreted. Endosome. Lysosome.Cell surface. Endoplasmic reticulum. Golgi apparatus.Autocatalytic cleavage is required to transport it from theendoplasmic reticulum to the Golgi apparatus and for the secretionof the mature protein.
应用详情
sensitivity:10pg ml
Detect Range:156pg ml-10 000pg ml
sample_type:cell culture supernates cell lysates tissue homogenates serum and plasma (heparin EDTA).
capture_antibody:monoclonal antibody from mouse
detection_antibody:polyclonal antibody from goat
gene_name:PCSK9
protein_name:Proprotein convertase subtilisin kexin type 9
gene_full_name:Proprotein convertase subtilisin kexin type 9
tissue_specificity: Expressed in neuro-epithelioma coloncarcinoma hepatic and pancreatic cell lines and in Schwanncells.
sequence_similarities:Belongs to the peptidase S8 family.
tmb_incubation:20-25min
research_category:signal transduction|metabolism|amino acids|cardiovascular|lipids / lipoproteins|lipid metabolism|cholesterol metabolism|stem cells|neural stem cells|intracellular|cell biology|proteolysis / ubiquitin|proteolytic enzymes|serine protease|proprotein convertases|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|amino acid metabolism|types of disease|cancer
产品描述
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human PCSK9
背景
protein_function: Crucial player in the regulation of plasma cholesterolhomeostasis. Binds to low-density lipid receptor family members:low density lipoprotein receptor (LDLR), very low densitylipoprotein receptor (VLDLR), apolipoprotein E receptor(LRP1,APOER) and apolipoprotein receptor 2 (LRP8,APOER2), andpromotes their degradation in intracellular acidic compartments(PubMed:18039658). Acts via a non-proteolytic mechanism to enhancethe degradation of the hepatic LDLR through a clathrinLDLRAP1,ARH-mediated pathway. May prevent the recycling of LDLRfrom endosomes to the cell surface or direct it to lysosomes fordegradation. Can induce ubiquitination of LDLR leading to itssubsequent degradation (PubMed:18799458, PubMed:17461796,PubMed:18197702, PubMed:22074827). Inhibits intracellulardegradation of APOB via the autophagosome,lysosome pathway in aLDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway(PubMed:18660751). Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expressionprimarily by increasing its proteasomal degradation. Regulatesneuronal apoptosis via modulation of LRP8,APOER2 levels andrelated anti-apoptotic signaling pathways..
Proprotein convertase subtilisin,kexin type 9, also known as PCSK9, is an enzyme that in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. By genomic sequence analysis, the PCSK9 gene was mapped to chromosome 1p32. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A(EGF-A) domain of the low-density lipoprotein receptor(LDLR), inducing LDLR degradation. Reduced LDLR levels result in decreased metabolism of low-density lipoproteins(LDL), which could lead to hypercholesterolemia. PCSK9 may also have a role in the differentiation of cortical neurons.
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