产品详情
产品名称DOPEY2 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Immunogen affinity purified
应用WB, ICC, IHC, FC
种属反应性Hu, Ms
免疫原描述Peptide
别名Dipeptidase 2 antibody
Dipeptidase2 antibody
DPEP 2 antibody
MBD 2 antibody
MBD2 antibody
数据库入口号Swiss-Prot#:Q9Y3R5
计算分子量260kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of Dopey-2 on mouse heart (1) and human heart (2) tissue lysate using anti-Dopey-2 antibody at 1/500 dilution.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse heart tissue labeling Dopey-2. Counterstained with Hematoxylin.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse brain tissue labeling Dopey-2. Counterstained with Hematoxylin.
ICC staining Dopey-2 in D3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of N2A cells with Dopey-2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
背景
Dopey-2, a 2,298 amino acid protein, belongs to the dopey family, that is overexpressed in lymphoblasts from Down syndrome patients and Abundantly expressed in developing central nervous system, with highest levels in cerebellum and lowest in telencephalon. Multiple isoforms of Dopey-2 exist due to alternative splicing events. Dopey-2 is thought to play a role in protein trafficking between early endosomes and the late.
背景文献
1.Rachidi M et al. A quantitative assessment of gene expression (QAGE) reveals differential overexpression of DOPEY2, a candidate gene for mental retardation, in Down syndrome brain regions. Int J Dev Neurosci 27(4):393-8 (2009).
2.Swaminathan S et al. Analysis of copy number variation in Alzheimer's disease: the NIALOAD/ NCRAD Family Study. Curr Alzheimer Res 9(7):801-14 (2012).
Yes
