产品详情
产品名称VDAC1 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Immunogen affinity purified
应用WB, ICC, IHC
种属反应性Hu, zebrafish
免疫原描述peptide
别名hVDAC1 antibody Outer mitochondrial membrane protein porin 1 antibody Plasmalemmal porin antibody Porin 31HL antibody Porin 31HM antibody Porin antibody VDAC 1 antibody VDAC antibody VDAC-1 antibody Vdac1 antibody VDAC1_HUMAN antibody Voltage Dependent Anion Channel 1 antibody Voltage dependent anion selective channel protein 1 antibody Voltage-dependent anion-selective channel protein 1 antibody
数据库入口号Swiss-Prot#:P21796
计算分子量31 kDa
配方1*TBS (pH7.4), 0.5%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500
IHC: 1:200
ICC: 1:200
Western blot analysis on different cell lysates using anti-VDAC1 rabbit polyclonal antibodies.
Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-VDAC1 rabbit polyclonal antibody.
ICC staining VDAC1 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
背景
Voltage-dependent anion-selective channel (VDAC1) (also referred to as porin, isoform 1) is a small protein, originally discovered in the outer membrane of mitochondria where it constitutes the major pore-forming protein. The porin protein VDAC1 allows to the outer-most membrane of the mitochondrion free permeability to low molecular-weight solutes. VDAC1 has been shown to co-immunoprecipitate with the anti-apoptotic protein Bcl-2 and suggested to be involved in forming the mitochondrial pore which releases cytochrome c during apoptosis.
背景文献
1."Influenza virus PB1-F2 protein induces cell death through mitochondrial ANT3 and VDAC1."Zamarin D., Garcia-Sastre A., Xiao X., Wang R., Palese P. PLoS Pathog. 1:40-54(2005) 2."Solution structure of the integral human membrane protein VDAC-1 in detergent micelles." Hiller S., Garces R.G., Malia T.J., Orekhov V.Y., Colombini M., Wagner G. Science 321:1206-1210(2008)
Yes
