产品详情
产品名称JNK Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Immunogen affinity purified
应用WB, IP, IF, FCM
种属反应性Hu, Ms, Rt
免疫原描述Amino acids 1-384 representing full length JNK1 of human origin.
别名AI849689 antibody c Jun N terminal kinase 1 antibody C-JUN kinase 1 antibody c-Jun N-terminal kinase 1 antibody EC 2.7.11.24 antibody JNK 1 antibody JNK antibody JNK-46 antibody JNK1A2 antibody JNK21B1/2 antibody MAP kinase 8 antibody MAPK 8 antibody mapk8 antibody Mitogen activated protein kinase 8 antibody Mitogen-activated protein kinase 8 antibody MK08_HUMAN antibody p54 gamma antibody Prkm8 antibody Protein kinase JNK1 antibody Protein kinase, mitogen-activated, 8 antibody SAPK 1 antibody SAPK gamma antibody SAPK1 antibody Stress-activated protein kinase 1 antibody Stress-activated protein kinase JNK1 antibody
数据库入口号Swiss-Prot#:P45983
计算分子量46/54kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:100-1:1,000
IP: 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)
FC: 1 μg per 1 x 106 cells
Western blot analysis of JNK phosphorylation in untreated (A,D), anisomycin treated (B,E) and anisomycin and lambda protein phosphatasetreated (C,F) Jurkat whole cell lysates.
Western blot analysis of JNK p46 and JNK p54 expression in K-562 (A), A-431 (B), NIH/3T3 (C), HeLa (D), RAW264.7 (E) and Jurkat (F) whole cell lysates.
背景
c-Jun N-terminal kinases (JNKs) phosphorylate and augment transcriptional activity of c-Jun. JNKs originate from three genes that yield ten isoforms through alternative mRNA splicing, including JNK1α1, JNK1β1, JNK2α1, JNK2β1 and JNK3α1, which represent the p46 isoforms, and JNK1α2, JNK1β2, JNK2α2, JNK2β2 and JNK3β2, which represent the p54 isoforms. JNKs coordinate cell responses to stress and influence regulation of cell growth and transformation. The human JNK1 (PRKM8, SAPK1, MAPK8) gene maps to chromosome 10q11.22 and shares 83% amino acid identity with JNK2. JNK1 is necessary for normal activation and differentiation of CD4 helper T (TH) cells into TH1 and TH2 effector cells. Capsaicin activates JNK1 and p38 in Ras-transformed human breast epithelial cells. Nitrogen oxides (NOx) upregulate JNK1 in addition to c-Fos, c-Jun and other signaling kinases, including MEKK1 and p38.
背景文献
1. Uchida, Y., et al. 2012. Involvement of stress kinase mitogen-activated protein kinase kinase 7 in regulation of mammalian circadian clock. J. Biol. Chem. 287: 8318-8326. 2. Sun, P.H., et al. 2012. Protein tyrosine phosphatase μ (PTP μ or PTPRM), a negative regulator of proliferation and invasion of breast cancer cells, is associated with disease prognosis. PLoS ONE 7: e50183.
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