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Sequestosome-1 Antibody#48357

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Sequestosome-1 Antibody
Sequestosome-1 Antibody
Sequestosome-1 Antibody
Sequestosome-1 Antibody
Sequestosome-1 Antibody
Sequestosome-1 Antibody
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产品详情

产品名称Sequestosome-1 Antibody

来源种属Mouse

克隆号10-E10

纯化Peptide affinity purified.

应用ICC，IHC,FC

种属反应性Hu, Ms, Rt

免疫原描述Synthetic peptide (KLH-coupled) within human SQSTM1 40-100 aa.

别名A170 antibody
DMRV antibody
EBI 3 associated protein of 60 kDa antibody
EBI 3 associated protein p60 antibody
EBI3 associated protein of 60 kDa antibody
EBI3 associated protein p60 antibody
EBI3-associated protein of 60 kDa antibody
EBIAP antibody
FTDALS3 antibody
MGC127197 antibody
ORCA antibody
OSF-6 antibody
Osi antibody
OSIL antibody
Oxidative stress induced like antibody
p60 antibody
p62 antibody
p62B antibody
Paget disease of bone 3 antibody
PDB 3 antibody
PDB3 antibody
Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa antibody
Phosphotyrosine independent ligand for the Lck SH2 domain p62 antibody
Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa antibody
PKC-zeta-interacting protein antibody
Protein kinase C-zeta-interacting protein antibody
Sequestosome 1 antibody
Sequestosome-1 antibody
SQSTM 1 antibody
SQSTM_HUMAN antibody
Sqstm1 antibody
STAP antibody
STONE14 antibody
Ubiquitin binding protein p62 antibody
Ubiquitin-binding protein p62 antibody
ZIP 3 antibody
ZIP antibody
ZIP3 antibody

数据库入口号Swiss-Prot#:Q13501

计算分子量62 kDa

配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

保存Store at -20˚C

应用详情

WB: 1:500-1:1,000
IHC: 1:200
ICC: 1:200

FC: 1 μg per 1 x 106 cells

Western blot analysis of SQSTM1?on different cell lysates using anti-SQSTM1 antibody at 1/500 dilution. Positive control: </br>Lane1: Hela </br>Lane2: HepG2

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti- SQSTM1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti- SQSTM1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-SQSTM1 antibody. Counter stained with hematoxylin.

ICC staining SQSTM1 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Flow cytometric analysis of Hela?cells with SQSTM1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.

背景

Sequestosome 1 (SQSTM1, p62) is a ubiquitin binding protein involved in cell signaling, oxidative stress and autophagy. It was first identified as a protein that binds to the SH2 domain of p56Lck and independently found to interact with PKCζ. SQSTM1 was subsequently found to interact with ubiquitin, providing a scaffold for several signaling proteins and triggering degradation of proteins through the proteasome or lysosome. Interaction between SQSTM1 and TRAF6 leads to the K63-linked polyubiquitination of TRAF6 and subsequent activation of the NF-κB pathway. It may play a role in titin/TTN downstream signaling in muscle cells and regulate signaling cascades through ubiquitination. P62 may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels.

背景文献

1."The atypical PKC-interacting protein p62 channels NF-kappaB activation by the IL-1-TRAF6 pathway." Sanz L., Diaz-Meco M.T., Nakano H., Moscat J. EMBO J. 19:1576-1586(2000)
2."Sequestosome 1/p62 is a polyubiquitin chain binding protein involved in ubiquitin proteasome degradation." Seibenhener M.L., Babu J.R., Geetha T., Wong H.C., Krishna N.R., Wooten M.W. Mol. Cell. Biol. 24:8055-8068(2004)
3."Identification of SQSTM1 mutations in familial Paget's disease in Australian pedigrees." Good D.A., Busfield F., Fletcher B.H., Lovelock P.K., Duffy D.L., Kesting J.B., Andersen J., Shaw J.T.E.Bone 35:277-282(2004)

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5

操作手册

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu人
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk猴
  • Rb兔
  • B牛
  • D狗
  • P猪
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk鸡

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025-58868422

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