产品详情
产品名称ZBTB48 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Peptide affinity purified
应用WB,ICC,IHC,FC
种属反应性Hu, Ms, Rt
免疫原描述Peptide
别名0610011D15Rik antibody
AI327031 antibody
GLI Kruppel family member HKR3 antibody
HKR3 antibody
Krueppel related zinc finger protein 3 antibody
Krueppel-related zinc finger protein 3 antibody
OTTMUSP00000031803 antibody
pp9964 antibody
Protein HKR3 antibody
RP23-445E20.3 antibody
ZBT48_HUMAN antibody
ZBTB 48 antibody
Zbtb48 antibody
Zinc finger and BTB domain containing 48 antibody
Zinc finger and BTB domain containing protein 48 antibody
Zinc finger and BTB domain-containing protein 48 antibody
Zinc finger protein 855 antibody
ZNF855 antibody
数据库入口号Swiss-Prot#:P10074
计算分子量77 kDa
配方1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:200
Western blot analysis of ZBTB48 on mouse spleen tissue lysate using anti-ZBTB48 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ZBTB48 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ZBTB48 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ZBTB48 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ZBTB48 antibody. Counter stained with hematoxylin.
ICC staining ZBTB48 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ZBTB48 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with ZBTB48 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
背景
Telomere-binding protein that acts as a regulator of telomere length. Directly binds the telomeric double-stranded 5'-TTAGGG-3' repeat. Preferentially binds to telomeres that have a low concentration of shelterin complex and acts as a regulator of telomere length by initiating telomere trimming, a process that prevents the accumulation of aberrantly long telomeres. Also acts as a transcription regulator that binds to promoter regions. Regulates expression of a small subset of genes, including MTFP1. Regulates expression the J and/or S elements in MHC II promoter. Acts as a negative regulator of cell proliferation by specifically activating expression of ARF, a tumor suppressor isoform of CDKN2A.
背景文献
1. Sugawara M et al. A factor that regulates the class II major histocompatibility complex gene DPA is a member of a subfamily of zinc finger proteins that includes a Drosophila developmental control protein. Mol Cell Biol 14:8438-8450 (1994).
2. Yoon J H et al. Human Kruppel-related 3 (HKR3) is a novel transcription activator of alternate reading frame (ARF) gene. J Biol Chem 289:4018-4031 (2014).
Yes
