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位置: 首页 > 抗体 > Vitamin D3 Receptor Antibody

Vitamin D3 Receptor Antibody#48553

Vitamin D3 Receptor Antibody
Vitamin D3 Receptor Antibody
Vitamin D3 Receptor Antibody
Vitamin D3 Receptor Antibody
Vitamin D3 Receptor Antibody
是否有货: Yes
产品总价:
产品详情

产品名称Vitamin D3 Receptor Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Protein affinity purified

应用WB,ICC,FC

种属反应性Hu, Rt

免疫原描述Recombinant protein with human Vitamin D Receptor aa 100-300.

别名1 25 dihydroxyvitamin D3 receptor antibody
1 antibody
1,25 dihydroxyvitamin D3 receptor antibody
1,25-@dihydroxyvitamin D3 receptor antibody
25-dihydroxyvitamin D3 receptor antibody
Member 1 antibody
NR1I1 antibody
Nuclear receptor subfamily 1 group I member 1 antibody
PPP1R163 antibody
Protein phosphatase 1, regulatory subunit 163 antibody
VDR antibody
VDR_HUMAN antibody
Vitamin D (1,25- dihydroxyvitamin D3) receptor antibody
Vitamin D hormone receptor antibody
Vitamin D nuclear receptor variant 1 antibody
Vitamin D receptor antibody
Vitamin D3 receptor antibody

数据库入口号Swiss-Prot#:P11473

计算分子量48 kDa

配方1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.

保存Store at -20˚C

应用详情
WB: 1:1,000-1:2,000

ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of Vitamin D Receptor on different cell lysate using anti-Vitamin D Receptor antibody at 1/2,000 dilution.
Positive control��
Lane1: U937
Lane2: SK-Br-3
ICC staining Vitamin D Receptor in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Vitamin D Receptor in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Vitamin D Receptor in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of LOVO cells with Vitamin D Receptor antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
背景
The active metabolite of vitamin D modulates the expression of a wide variety of genes in a developmentally specific manner. This secosteroid hormone can up- or downregulate the expression of genes involved in a diverse array of responses such as proliferation, differentiation and calcium homeostasis. 1,25-(OH)2-vitamin D3 exerts its effects through interaction with the vitamin D receptor (VDR), a member of the superfamily of hormone-activated nuclear receptors. In its ligand-bound state, the VDR forms heterodimers with the 9-cis retinoic acid receptor, RXR, and affects gene expression by binding specific DNA sequences known as hormone response elements, or HREs. In addition to regulating the above-mentioned cellular responses, 1,25-(OH)2-vitamin D3 exhibits antiproliferative properties in osteosarcoma, melanoma, colon carcinoma and breast carcinoma cells.
背景文献
1. Fujiki R et al. Ligand-induced transrepression by VDR through association of WSTF with acetylated histones. EMBO J 24:3881-3894 (2005).
2. Rochel N et al. The crystal structure of the nuclear receptor for vitamin D bound to its natural ligand. Mol Cell 5:173-179 (2000).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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025-58868422

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