产品详情
产品名称p53 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号SA39-07
纯化ProA affinity purified
应用WB, ICC/IF
种属反应性Hu, Zebrafish
免疫原描述recombinant protein
别名Antigen NY-CO-13 antibody
BCC7 antibody
Cellular tumor antigen p53 antibody
FLJ92943 antibody
LFS1 antibody
Mutant tumor protein 53 antibody
p53 antibody
p53 tumor suppressor antibody
P53_HUMAN antibody
Phosphoprotein p53 antibody
Tp53 antibody
Transformation related protein 53 antibody
TRP53 antibody
Tumor protein 53 antibody
Tumor protein p53 antibody
Tumor suppressor p53 antibody
数据库入口号Swiss-Prot#:P04637
计算分子量44/53 kDa (Human), 41kDa (Zebrafish)
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-5,000
ICC: 1:50-1:200
Western blot analysis of p53 on different cell lysates using anti-p53 antibody at 1/1,000 dilution. Positive control:
Lane 1: 293T
Lane 2: 293
ICC staining p53 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining p53 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
背景
The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. P53 is found in increased amounts in a wide variety of transformed cells. P53 is frequently mutated or inactivated in about 60% of cancers.
背景文献
1. "WOX1 is essential for tumor necrosis factor-, UV light-, staurosporine-, and p53-mediated cell death, and its tyrosine 33-phosphorylated form binds and stabilizes serine 46-phosphorylated p53." Chang N.-S., Doherty J., Ensign A., Schultz L., Hsu L.-J., Hong Q. J. Biol. Chem. 280:43100-43108(2005).
2. "Protein kinase C delta regulates Ser46 phosphorylation of p53 tumor suppressor in the apoptotic response to DNA damage." Yoshida K., Liu H., Miki Y. J. Biol. Chem. 281:5734-5740(2006).
Yes
