产品详情
产品名称VCAM1 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号SA05-04
纯化ProA affinity purified
应用WB, ICC/IF, IHC, IP
种属反应性Hu, Ms, Rt, zebrafish
免疫原描述recombinant protein
别名CD106 antibody CD106 Antigen antibody INCAM 100 antibody INCAM-100 antibody L1CAM antibody MGC99561 antibody V-CAM 1 antibody Vascular Cell Adhesion Molecule 1 antibody Vascular cell adhesion protein 1 antibody VCAM 1 antibody VCAM-1 antibody VCAM1 antibody VCAM1_HUMAN antibody
数据库入口号Swiss-Prot#:P19320
计算分子量100 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200
Western blot analysis of VCAM1 on different cell lysates using anti-VCAM1 antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-VCAM1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-VCAM1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-VCAM1 antibody. Counter stained with hematoxylin.
ICC staining VCAM1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
背景
Cell adhesion molecules are a family of closely related cell surface glycoproteins involved in cell-cell interactions during growth and are thought to play an important role in embryogenesis and development. Neuronal cell adhesion molecule (NCAM) expression is observed in a variety of human tumors including neuroblastomas, rhabdomyosarcomas, Wilms' tumors, Ewing's sarcomas and some primitive myeloid malignancies. The intracellular adhesion molecule-1 (ICAM-1), also referred to as CD54, is an integral membrane protein of the immunoglobulin superfamily and recognizes the B2��1 and B2��M integrins. PECAM-1 (platelet/endothelial cell adhesion molecule-1), also referred to as CD31, is a glycoprotein expressed on the cell surfaces of monocytes, neutrophils, platelets and a subpopulation of T cells. VCAM-1 (vascular cell adhesion molecule-1) was first identified as an adhesion molecule induced on human endothelial cells by inflammatory cytokines such as IL-1, tumor necrosis factor (TNF) and lipopolysaccharide (LPS). The KALIG gene encodes a nerve cell adhesion molecule (NCAM) -like protein and is deleted in 66% of patients with Kallmann's syndrome, anosmia with secondary hypogonadism.
背景文献
1. Gregory EK et al. Periadventitial atRA citrate-based polyester membranes reduce neointimal hyperplasia and restenosis after carotid injury in rats. Am J Physiol Heart Circ Physiol 307:H1419-29 (2014). 2. Florea V et al. c-Myc Is Essential to Prevent Endothelial Pro-Inflammatory Senescent Phenotype. PLoS One 8:e73146 (2013).
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