产品详情
产品名称p75 NGF Receptor Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号SA39-02
纯化ProA affinity purified
应用WB, ICC/IF, IHC, IP
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名CD271 antibody CD271 antigen antibody Gp80 LNGFR antibody Gp80-LNGFR antibody Low affinity nerve growth factor receptor antibody Low affinity neurotrophin receptor p75NTR antibody Low-affinity nerve growth factor receptor antibody Nerve growth factor receptor antibody Nerve growth factor receptor TNFR superfamily member 16 antibody NGF receptor antibody Ngfr antibody p75 ICD antibody p75 Neurotrophin receptor antibody p75 NTR antibody p75(NTR) antibody p75NTR antibody TNFR Superfamily Member 16 antibody TNFRSF16 antibody TNR16_HUMAN antibody Tumor necrosis factor receptor superfamily member 16 antibody
数据库入口号Swiss-Prot#:P08138
计算分子量75 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200
Western blot analysis of NGFR p75 on human skeletal muscle lysates using anti-NGFR p75 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-NGFR p75 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-NGFR p75 antibody. Counter stained with hematoxylin.
ICC staining NGFR p75 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining NGFR p75 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
背景
The Trk oncogene encodes a membrane-spanning protein tyrosine kinase, gp140Trk, whose expression is restricted in vivo to neurons of the sensory spinal and cranial ganglia of neural crest origin. Nerve growth factor (NGF) stimulates tyrosine phosphorylation of Trk A in neural cell lines and in embryonic dorsal root ganglia. Tyrosine phosphorylation of Trk by NGF is rapid, specific and occurs with picomolar quantities of factor, indicating that the response is mediated by physiological amounts of NGF, suggesting that Trk A participates in the primary signal transduction mechanism of NGF. An additional component of the Trk A receptor complex, NGFR p75, binds to the neurotrophic factors with low affinity but is required for efficient signaling. NGFR p75 accelerates Trk A activation and may recruit downstream effector molecules to the liganded complex.
背景文献
1. Blanchard JW et al. Selective conversion of fibroblasts into peripheral sensory neurons. Nat Neurosci 18:25-35 (2015). 2. Fernandez-Enright F et al. Novel implications of Lingo-1 and its signaling partners in schizophrenia. Transl Psychiatry 4:e348 (2014).
Yes
