产品详情
产品名称Filamin A Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号SA30-08
纯化ProA affinity purified
应用WB, ICC/IF, IHC, FC
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名ABP 280 antibody ABP-280 antibody Actin-binding protein 280 antibody Alpha filamin antibody Alpha-filamin antibody APBX antibody CSBS antibody CVD1 antibody Endothelial actin binding protein antibody Endothelial actin-binding protein antibody Filamin 1 antibody Filamin A alpha antibody Filamin A antibody Filamin-1 antibody Filamin-A antibody FLN antibody FLN-A antibody FLN1 antibody FLNA antibody FLNA_HUMAN antibody FMD antibody MNS antibody NHBP antibody Non muscle filamin antibody Non-muscle filamin antibody OPD antibody OPD1 antibody OPD2 antibody XLVD antibody XMVD antibody
数据库入口号Swiss-Prot#:P21333
计算分子量281 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-5,000
IHC: 1:50-1:400
ICC: 1:50-1:200
FC: 1:10-1:100
Western blot analysis of Filamin A on different cell lysates using anti-Filamin A antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Jurkat Lane 3: Hela Lane 4: NIH/3T3
Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Filamin A antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-Filamin A antibody. Counter stained with hematoxylin.
ICC staining Filamin A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Filamin A in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Filamin A in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with Filamin A antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
背景
Caldesmon, Filamin 1, Nebulin and Villin are differentially expressed and regulated Actin binding proteins. Both muscular (CDh) and non-muscular (CDl) forms of Caldesmon have been identified and each has been shown to bind to Actin as well as to calmodulin and Myosin. CDh is expressed predominantly on thin filaments in smooth muscle, whereas CDl is widely expressed in non-muscle tissues and cells. Filamin 1, which is ubiquitously expressed and exists as a homodimer, functions to crosslink Actin to filaments. Nebulin is a large filamentous protein specific to muscle tissue that may function as a ruler for filament length. Several isoforms of Nebulin are produced by alternative exon usage. Villin is Ca2+-regulated and is the major structural component of the brush border of absorptive cells.
背景文献
1. Hu J et al. Filamin B regulates chondrocyte proliferation and differentiation through Cdk1 signaling. PLoS One 9:e89352 (2014). 2. Rafizadeh S et al. Functional interaction with filamin A and intracellular Ca2+ enhance the surface membrane expression of a small-conductance Ca2+-activated K+ (SK2) channel. Proc Natl Acad Sci U S A 111:9989-94 (2014).
Yes
