产品详情
产品名称Beta-Catenin Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号SA30-04
纯化ProA affinity purified
应用WB, ICC, IHC, IP
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名Beta catenin antibody
Beta-catenin antibody
Cadherin associated protein antibody
Catenin (cadherin associated protein), beta 1, 88kDa antibody
Catenin beta 1 antibody
Catenin beta-1 antibody
CATNB antibody
CHBCAT antibody
CTNB1_HUMAN antibody
CTNNB antibody
CTNNB1 antibody
DKFZp686D02253 antibody
FLJ25606 antibody
FLJ37923 antibody
OTTHUMP00000162082 antibody
OTTHUMP00000165222 antibody
OTTHUMP00000165223 antibody
OTTHUMP00000209288 antibody
OTTHUMP00000209289 antibody
数据库入口号Swiss-Prot#:P35222
计算分子量92 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:5,000
IHC: 1:20-1:200
ICC: 1:20-1:50
IP: 1:10-1:50
背景
β-Catenin is a key downstream effector in the Wnt signaling pathway. It is implicated in two major biological processes in vertebrates: early embryonic development and tumorigenesis. Phosphorylation at Ser-552 by AMPK promotes stabilizion of the protein, enhancing TCF/LEF-mediated transcription. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines.
背景文献
1. "AlphaT-catenin: a novel tissue-specific beta-catenin-binding protein mediating strong cell-cell adhesion."Janssens B., Goossens S., Staes K., Gilbert B., van Hengel J., Colpaert C., Bruyneel E., Mareel M., van Roy F. J. Cell Sci. 114:3177-3188(2001).
2. "Characterisation of the phosphorylation of beta-catenin at the GSK-3 priming site Ser45." Hagen T., Vidal-Puig A. Biochem. Biophys. Res. Commun. 294:324-328(2002).
Yes
