产品详情
产品名称DUSP6 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号SR39-09
纯化ProA affinity purified
应用WB, ICC/IF, IHC, IP, FC
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名Dual specificity phosphatase 6 antibody
Dual specificity phosphatase 6 isoform a antibody
Dual specificity protein phosphatase 6 antibody
Dual specificity protein phosphatase PYST1 antibody
DUS6_HUMAN antibody
DUSP 6 antibody
DUSP 6a antibody
Dusp6 antibody
DUSP6a antibody
HH19 antibody
MAP kinase phosphatase 3 antibody
Mitogen activated protein kinase phosphatase 3 antibody
Mitogen-activated protein kinase phosphatase 3 antibody
MKP 3 antibody
MKP-3 antibody
MKP3 antibody
PYST 1 antibody
PYST1 antibody
Serine/threonine specific protein phosphatase antibody
数据库入口号Swiss-Prot#:Q16828
计算分子量42 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of DUSP6 on mouse pancreas lysates using anti-DUSP6 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-DUSP6 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-DUSP6 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DUSP6 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-DUSP6 antibody. Counter stained with hematoxylin.
ICC staining DUSP6 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining DUSP6 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining DUSP6 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of NIH/3T3 cells with DUSP6 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
背景
Mitogen-activated protein (MAP) kinases are a large class of proteins involved in signal transduction pathways that are activated by a range of stimuli and mediate a number of physiological and pathological changes in the cell. Dual specificity phosphatases (DSPs) are a subclass of the protein tyrosine phosphatase (PTP) gene superfamily, which are selective for dephosphorylating critical phosphothreonine and phosphotyrosine residues within MAP kinases. DSP gene expression is induced by a host of growth factors and/or cellular stresses, thereby negatively regulating MAP kinase superfamily members including MAPK/ERK, SAPK/JNK and p38. The members of the dual-specificity phosphatase protein family include MKP-1/CL100 (3CH134), VHR, PAC1, MKP-2, hVH-3 (B23), hVH-5, MKP-3, MKP-X, and MKP-4. Human MKP-3 maps to chromosome 12q22-q23 and encodes a 381 amino acid protein that specifically inactivates members of the ERK family and is expressed in a variety of tissues with the highest levels in heart and pancreas.
背景文献
1. Bertin S et al. Dual-specificity phosphatase 6 regulates CD4(+) T-cell functions and restrains spontaneous colitis in IL-10-deficient mice. Mucosal Immunol N/A:N/A (2014).
2. Wales S et al. Global MEF2 target gene analysis in cardiac and skeletal muscle reveals novel regulation of DUSP6 by p38MAPK-MEF2 signaling. Nucleic Acids Res 42:11349-62 (2014).
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