产品详情
产品名称CCR7 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号SR36-04
纯化ProA affinity purified
应用WB, ICC/IF, IHC, IP
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名BLR 2 antibody BLR2 antibody C C chemokine receptor type 7 antibody C C CKR 7 antibody C-C chemokine receptor type 7 antibody C-C CKR-7 antibody CC chemokine receptor 7 antibody CC chemokine receptor type 7 antibody CC CKR 7 antibody CC-CKR-7 antibody CCCKR7 antibody CCR 7 antibody CCR-7 antibody Ccr7 antibody CCR7_HUMAN antibody CD 197 antibody CD197 antibody CD197 antigen antibody CDw197 antibody Chemokine (C C motif) receptor 7 antibody Chemokine (C C) receptor 7 antibody Chemokine C C motif receptor 7 antibody Chemokine C C receptor 7 antibody Chemokine receptor 7 antibody Chemokine receptor 7-like protein antibody CMKBR7 antibody EBI 1 antibody EBI1 antibody Ebi1h antibody EBV Induced G Protein Coupled Receptor 1 antibody EBV-induced G-protein coupled receptor 1 antibody Epstein Barr virus induced G protein coupled receptor antibody Epstein Barr virus induced gene 1 antibody Epstein-Barr virus-induced G-protein coupled receptor 1 antibody EVI 1 antibody EVI1 antibody Lymphocyte Specific G Protein Coupled Peptide Receptor antibody MGC108519 antibody MIP 3 beta receptor antibody MIP-3 beta receptor antibody MIP3 Beta Receptor antibody
数据库入口号Swiss-Prot#:P32248
计算分子量43 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200
Western blot analysis of CCR7 on different cell lysates using anti-CCR7 antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: PC-12 Lane 3: Daudi Lane 4: Jurkat
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CCR7 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CCR7 antibody. Counter stained with hematoxylin.
ICC staining CCR7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining CCR7 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
背景
C-C or �� chemokine family members are characterized by a pair of adjacent cysteine residues and serve as potent chemoattractants and activators of monocytes and T cells. C-C chemokine receptor family members include CKR-1, CKR-2A, CKR-2B, CKR-3, CKR-4, CKR-5, CKR-6, CKR-7, CKR-8, CKR-9, CKR-10 and the Duffy blood group antigen. Each of these receptors are G protein-coupled, seven pass transmembrane domain proteins whose major physiological role is to function in the chemotaxis of T cells and phagocytic cells to areas of inflammation. However, this receptor family has also been shown to facilitate viral infection. CKR-7 (C-C chemokine receptor type 7), also known as CCR7, CMKBR7, EBI1 or EVI1, is a 378 amino acid multi-pass membrane protein that belongs to the CC-chemokine receptor family. Expressed in activated B- and T-lymphocytes, as well as in various lymphoid tissues, CKR-7 functions as a receptor for MIP-3�� and is thought to be involved in mediating normal lymphocyte function.
背景文献
1. Pang MF et al. TGF-β1-induced EMT promotes targeted migration of breast cancer cells through the lymphatic system by the activation of CCR7/CCL21-mediated chemotaxis. Oncogene N/A:N/A (2015). 2. Guo J et al. Effect of CCR7, CXCR4 and VEGF-C on the lymph node metastasis of human pancreatic ductal adenocarcinoma. Oncol Lett 5:1572-1578 (2013).
Yes
