产品详情
产品名称MMP12 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号SR03-23
纯化ProA affinity purified
应用WB, ICC/IF, IHC, IP, FC
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名EC 3.4.24.65 antibody
HME antibody
Macrophage elastase antibody
Macrophage metalloelastase antibody
Macrophage metaloelastase antibody
Matrix metallopeptidase 12 (macrophage elastase) antibody
Matrix metalloprotease 12 antibody
Matrix metalloproteinase-12 antibody
ME antibody
MGC138506 antibody
MME antibody
MMP 12 antibody
MMP-12 antibody
Mmp12 antibody
MMP12_HUMAN antibody
数据库入口号Swiss-Prot#:P39900
计算分子量54/45/22 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of MMP12 on different cell lysates using anti-MMP12 antibody at 1/1,000 dilution. Positive control:
Lane 1: A549
Lane 2: Hela
Lane 3: MCF-7
Lane 4: THP-1
Immunohistochemical analysis of paraffin-embedded human breast carcinomna tissue using anti-MMP12 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-MMP12 antibody. Counter stained with hematoxylin.
Flow cytometric analysis of A549 cells with MMP12 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
背景
The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-12 (also designated macrophage metalloelastase) is produced in alveolar macrophages and degrades elastin. MMP-12 may contribute to elastin degradation occurring in granulomatous skin diseases and may also participate in macrophage migration through the epidermal and vascular basement membranes in inflammatory disorders.
背景文献
1. John-Schuster G et al. Cigarette smoke-induced iBALT mediates macrophage activation in a B cell-dependent manner in COPD. Am J Physiol Lung Cell Mol Physiol 307:L692-706 (2014).
2. Stawski L et al. MMP-12 deficiency attenuates angiotensin II-induced vascular injury, M2 macrophage accumulation, and skin and heart fibrosis. PLoS One 9:e109763 (2014).
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