产品详情
产品名称VEGF Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号SP07-01
纯化ProA affinity purified
应用ICC/IF, IHC, IP, FC
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名Folliculostellate cell-derived growth factor antibody Glioma-derived endothelial cell mitogen antibody MGC70609 antibody MVCD1 antibody Vascular endothelial growth factor A antibody vascular endothelial growth factor A121 antibody vascular endothelial growth factor A165 antibody vascular endothelial growth factor antibody Vascular permeability factor antibody VEGF A antibody Vegf antibody VEGF-A antibody VEGF120 antibody Vegfa antibody VEGFA_HUMAN antibody VPF antibody
数据库入口号Swiss-Prot#:P15692
计算分子量43 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
IHC:1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-VEGF antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcimoma tissue using anti-VEGF antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-VEGF antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-VEGF antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-VEGF antibody. Counter stained with hematoxylin.
ICC staining VEGF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining VEGF in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining VEGF in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining VEGF in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with VEGF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
背景
The onset of angiogenesis is believed to be an early event in tumorigenesis and may facilitate tumor progression and metastasis. Several growth factors with angiogenic activity have been described. These include fibroblast growth factors (FGFs), platelet derived growth factor (PDGF) and vascular endothelial growth factor (VEGF). VEGF is a dimeric glycoprotein with structural homology to PDGF. Several variants of VEGF have been described that arise by alternative mRNA splicing. It has been speculated that VEGF may function as a tumor angiogenesis factor in vivo because the expression pattern of VEGF is consistent with a role in embryonic angiogenesis. VEGF mRNA is formed in some primary tumors, VEGF is produced by tumor cell lines in vitro and VEGF mitogenic activity appears to be restricted to endothelial cells. A member of the PDGF receptor family, Flt, has been identified as a high-affinity receptor for VEGF.
背景文献
1. Zhao L et al. Effect of Chronic Psychological Stress on Liver Metastasis of Colon Cancer in Mice. PLoS One 10:e0139978 (2015). 2. Qi JS et al. Combination of interventional adenovirus-p53 introduction and ultrasonic irradiation in the treatment of liver cancer. Oncol Lett 9:1297-1302 (2015).
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