产品详情
产品名称VEGF Receptor 1 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号SY09-09
纯化ProA affinity purified
应用WB, ICC/IF, IHC, IP, FC
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名EC 2.7.10.1 antibody FLT 1 antibody FLT antibody Flt-1 antibody FLT1 antibody Fms like tyrosine kinase 1 antibody Fms related tyrosine kinase 1 antibody Fms related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) antibody Fms related tyrosine kinase 1 vascular endothelial growth factor/vascular permeability factor receptor antibody Fms-like tyrosine kinase 1 antibody FRT antibody Soluble VEGF receptor 1 14 antibody Soluble VEGFR1 variant 2 antibody Soluble VEGFR1 variant 21 antibody Tyrosine protein kinase FRT antibody Tyrosine protein kinase receptor FLT antibody Tyrosine-protein kinase FRT antibody Tyrosine-protein kinase receptor FLT antibody Vascular endothelial growth factor receptor 1 antibody Vascular endothelial growth factor vascular permeability factor receptor antibody Vascular permeability factor receptor 1 antibody Vascular permeability factor receptor antibody VEGFR 1 antibody VEGFR-1 antibody VEGFR1 antibody VGFR1_HUMAN antibody
数据库入口号Swiss-Prot#:P17948
计算分子量151 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of VEGF Receptor 1 on mouse lung lysates using anti-VEGF Receptor 1 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-VEGF Receptor 1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-VEGF Receptor 1 antibody. Counter stained with hematoxylin.
ICC staining VEGF Receptor 1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining VEGF Receptor 1 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining VEGF Receptor 1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of A431 cells with VEGF Receptor 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
背景
Three cell membrane receptor tyrosine kinases, Flt (also designated VEGF-R1), Flk-1 (also designated VEGF-R2) and Flt-4, putatively involved in the growth of endothelial cells, are characterized by the presence of seven immunoglobulin-like sequences in their extracellular domain. These receptors exhibit high degrees of sequence relatedness to each other as well as lesser degrees of relatedness to the class III receptors including CSF-1/Fms, PDGR, SLFR/Kit and Flt-3/Flk-2. Two members of this receptor class, Flt-1 and Flk-1, have been shown to represent high affinity receptors for vascular endothelial growth factors (VEGFs). On the basis of structural similarity to Flt and Flk-1, it has been speculated that Flt-4 might represent a third receptor for either VEGF or a VEGF-related ligand.
背景文献
1. Zhou W et al. Fibroblast growth factor receptor 1 promotes MG63 cell proliferation and is associated with increased expression of cyclin-dependent kinase 1 in osteosarcoma. Mol Med Rep 13:713-9 (2016). 2. Liu X et al. Impaired VEGF Signaling in Lungs with Hypoplastic Esophageal Atresia and Effects on Branching Morphogenesis. Cell Physiol Biochem 39:385-94 (2016).
Yes
