产品详情
产品名称CD8 alpha Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号SI18-01
纯化ProA affinity purified
应用WB, ICC/IF, IHC
种属反应性Hu
免疫原描述recombinant protein
别名alpha polypeptide (p32) antibody CD8 antibody CD8 antigen alpha polypeptide antibody CD8 antigen alpha polypeptide (p32) antibody CD8a antibody CD8A antigen antibody CD8A molecule antibody CD8A_HUMAN antibody Leu2 antibody Leu2 T lymphocyte antigen antibody Ly3 antibody LYT3 antibody MAL antibody OKT8 T cell antigen antibody OTTHUMP00000160760 antibody OTTHUMP00000160764 antibody OTTHUMP00000203528 antibody OTTHUMP00000203721 antibody p32 antibody T cell antigen Leu2 antibody T cell co receptor antibody T-cell surface glycoprotein CD8 alpha chain antibody T-lymphocyte differentiation antigen T8/Leu-2 antibody T8 T cell antigen antibody T8/Leu-2 T-lymphocyte differentiation antigen antibody
数据库入口号Swiss-Prot#:P01732
计算分子量26 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
Western blot analysis of CD8 alpha on different lysates using anti-CD8 alpha antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Lovo
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD8 alpha antibody. Counter stained with hematoxylin.
ICC staining CD8 alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining CD8 alpha in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
背景
The T cell receptor (TCR) is a heterodimer composed of either α and β or γ and δ chains. CD3 chains and the CD4 or CD8 (CD8-α and CD8-β) co-receptors are also required for efficient signal transduction through the TCR. The TCR is expressed on T helper and T cytotoxic cells that can be distinguished by their expression of CD4 and CD8 proteins; T helper cells express CD4 proteins and T cytotoxic cells display CD8 proteins. CD8s are cell surface glycoproteins that exist as two chain complex (αα or αβ) receptors that bind class I MHC molecules presented by the antigen-presenting cell (APC). A primary function of CD8 proteins is to facilitate antigen recognition by the TCR and to strengthen the avidity of the TCR-antigen interactions. An additional role for CD8-expressing T cells may be to maintain low levels of HIV expression.
背景文献
1. Zehbe, I. et al. 2007. Human papillomavirus 16 E6-specific CD45RA+ CCR7+ high avidity CD8+ T cells fail to control tumor growth despite interferon-gamma production in patients with cervical cancer. J Immunother. 30: 523-532. 2. Morales, P.J. et al. 2003. Placental cell expression of HLA-G2 isoforms is limited to the invasive trophoblast phenotype. J. Immunology. 171: 6215-6224.
Yes
