Yes
Western blot analysis of CD86 on different lysates using anti-CD86 antibody at 1/1,000 dilution. Positive control:
Lane 1: Raji
Lane 2: Jurkat
Lane 1: Raji
Lane 2: Jurkat
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD86 antibody. Counter stained with hematoxylin.
ICC staining CD86 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining CD86 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining CD86 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of K562 cells with CD86 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
