免费电话:400-9961-933
邮箱:techcn@signalwayantibody.com
欢迎光临本站
登录 注册 English
位置: 首页 > 重组兔单抗 > DARPP32 Rabbit mAb

DARPP32 Rabbit mAb#48815

评价
Recombinant
DARPP32 Rabbit mAb
DARPP32 Rabbit mAb
DARPP32 Rabbit mAb
DARPP32 Rabbit mAb
DARPP32 Rabbit mAb
DARPP32 Rabbit mAb
DARPP32 Rabbit mAb
是否有货: Yes
产品总价:
产品详情

产品名称DARPP32 Rabbit mAb

来源种属Recombinant Rabbit

克隆性 Monoclonal antibody

克隆号SU0329

纯化ProA affinity purified

应用WB, ICC/IF, IHC, IP, FC

种属反应性Hu, Ms, Rt

免疫原描述recombinant protein

别名DARPP 32 antibody
DARPP-32 antibody
Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody
Dopamine and cAMP regulated neuronal phosphoprotein antibody
Dopamine and cAMP regulated phosphoprotein antibody
Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody
Dopamine and cAMP regulated phosphoprotein DARPP32 antibody
Dopamine- and cAMP-regulated neuronal phosphoprotein antibody
FLJ20940 antibody
IPPD antibody
Neuronal phosphoprotein DARPP 32 antibody
PPP1R1B antibody
PPR1B_HUMAN antibody
Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody
Protein phosphatase 1 regulatory inhibitor subunit 1B antibody
Protein phosphatase 1 regulatory subunit 1B antibody

数据库入口号Swiss-Prot#:Q9UD71

计算分子量32 kDa

配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

保存Store at -20˚C

应用详情
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of DARPP32 on mouse brain lysates using anti-DARPP32 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-DARPP32 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-DARPP32 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-DARPP32 antibody. Counter stained with hematoxylin.
ICC staining DARPP32 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining DARPP32 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with DARPP32 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
背景
Dopaminergic signaling pathways, which are essential for multiple brain functions, are abnormal in several neurological disorders, such as schizophrenia, Parkinson's disease and drug abuse. DARPP-32 (for dopamine and adenosine 3',5'-monophosphate-regulated phosphoprotein) is abundant in neurons that receive dopaminergic input. Activation of PKA and the consequent phosphorylation of DARPP-32 on threonine occurs in response to dopamine acting upon D1-like receptors. Dopamine interaction with D2-like receptors results in the inhibition of PKA activation, the activation of protein phosphatase 2B and the consequent dephosphorylation of DARPP-32. Neurotransmitters other than dopamine may also be able to stimulate the phosphorylation or dephosphorylation of DARPP-32. Phosphorylated DARPP-32 is a potent inhibitor of PP-1.
背景文献
1. Murata K et al. Mapping of Learned Odor-Induced Motivated Behaviors in the Mouse Olfactory Tubercle. J Neurosci 35:10581-99 (2015).
2. Lewitus GM et al. An adaptive role of TNFa in the regulation of striatal synapses. J Neurosci 34:6146-55 (2014).

如果您使用该产品48815发表了文章,请通知我们,让我们可以引用您的文献。

5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

Location :

南京市高新开发区星火路10号人才大厦C座

Phone :

400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com