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位置: 首页 > 重组兔单抗 > Phospho-Nrf2(S40) Rabbit mAb

Phospho-Nrf2(S40) Rabbit mAb#13360

评价
Recombinant
Phospho-Nrf2(S40) Rabbit mAb
Phospho-Nrf2(S40) Rabbit mAb
Phospho-Nrf2(S40) Rabbit mAb
Phospho-Nrf2(S40) Rabbit mAb
Phospho-Nrf2(S40) Rabbit mAb
Phospho-Nrf2(S40) Rabbit mAb
Phospho-Nrf2(S40) Rabbit mAb
Phospho-Nrf2(S40) Rabbit mAb
是否有货: Yes
产品总价:
产品详情

产品名称Phospho-Nrf2(S40) Rabbit mAb

来源种属Rabbit

克隆性Monoclonal

克隆号SU0334

纯化Affinity-chromatography

应用WB,IHC,ICC/IF,IP,FC

种属反应性Hu

免疫原描述A synthesized peptide derived from human Phospho-Nrf2 (S40)

别名erythroid derived 2 antibody
HEBP1 antibody
like 2 antibody
NF E2 related factor 2 antibody
NF-E2-related factor 2 antibody
NF2L2_HUMAN antibody
NFE2 related factor 2 antibody
NFE2-related factor 2 antibody
Nfe2l2 antibody
Nrf 2 antibody
NRF2 antibody
Nuclear factor (erythroid derived 2) like 2 antibody
Nuclear factor antibody
nuclear factor erythroid 2 like 2 antibody
Nuclear factor erythroid 2 related factor 2 antibody
Nuclear factor erythroid 2-related factor 2 antibody
Nuclear factor erythroid derived 2 like 2 antibody

数据库入口号Swiss-Prot#:Q16236

计算分子量90 kDa

配方Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情

WB 1:1000~1:2000
IHC 1:50~1:200
ICC/IF 1:50~1:200
IP 1:50 FC 1:50

Western blot analysis of Phospho-Nrf2(S40) on different lysates using anti-Phospho-Nrf2(S40) antibody at 1/1,000 dilution. Positive control:
Lane 1: HepG2
Lane 2: Raji
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-Nrf2(S40) antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Nrf2(S40) antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Phospho-Nrf2(S40) antibody. Counter stained with hematoxylin.
ICC staining Phospho-Nrf2(S40) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Phospho-Nrf2(S40) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Phospho-Nrf2(S40) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of K562 cells with Phospho-Nrf2(S40) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
背景

Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.

背景文献

1. Masuda Y et al. The effect of Nrf2 pathway activation on human pancreatic islet cells. PLoS One 10:e0131012 (2015).
2. Stachel I et al. Modulation of nuclear factor E2-related factor-2 (Nrf2) activation by the stress response gene immediate early response-3 (IER3) in colonic epithelial cells: a novel mechanism of cellular adaption to inflammatory stress. J Biol Chem 289:1917-29 (2014).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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