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Lactate Dehydrogenase Rabbit mAb#48839

评价
Recombinant
Lactate Dehydrogenase Rabbit mAb
Lactate Dehydrogenase Rabbit mAb
Lactate Dehydrogenase Rabbit mAb
Lactate Dehydrogenase Rabbit mAb
Lactate Dehydrogenase Rabbit mAb
Lactate Dehydrogenase Rabbit mAb
Lactate Dehydrogenase Rabbit mAb
Lactate Dehydrogenase Rabbit mAb
Lactate Dehydrogenase Rabbit mAb
Lactate Dehydrogenase Rabbit mAb
是否有货: Yes
产品总价:
产品详情

产品名称Lactate Dehydrogenase Rabbit mAb

克隆号SU39-06

纯化ProA affinity purified

应用WB, ICC/IF, IHC, IP, FC

种属反应性Hu, Ms, Rt, zebrafish

免疫原描述recombinant protein

别名Cell proliferation-inducing gene 19 protein antibody
GSD11 antibody
L lactate dehydrogenase B chain antibody
L-lactate dehydrogenase A chain antibody
Lactate dehydrogenase A antibody
Lactate dehydrogenase B antibody
Lactate dehydrogenase H chain antibody
Lactate dehydrogenase M antibody
LDH A antibody
LDH B antibody
LDH H antibody
LDH heart subunit antibody
LDH M antibody
LDH muscle subunit antibody
LDH-A antibody
LDH-M antibody
LDH1 antibody
ldha antibody
LDHA_HUMAN antibody
LDHBD antibody
LDHM antibody
MS1111 antibody
PIG19 antibody
Proliferation inducing gene 19 antibody
Renal carcinoma antigen NY REN 46 antibody
Renal carcinoma antigen NY-REN-59 antibody
TRG 5 antibody
TRG5 antibody

数据库入口号Swiss-Prot#:P00338

计算分子量37 kDa

浓度1mg/ml

配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

保存Store at -20˚C

应用详情

WB: 1:1,000-5,000
IHC: 1:200-1:500
ICC: 1:50-1:200

FC: 1:50-1:100

Western blot analysis of Lactate Dehydrogenase on different lysates using anti-Lactate Dehydrogenase antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: A549
Lane 3: MCF-7
Western blot analysis of Lactate Dehydrogenase on hybrid fish (crucian-carp) brain tissue lysate using anti-Lactate Dehydrogenase antibody at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.
ICC staining Lactate Dehydrogenase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Lactate Dehydrogenase in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
背景

The lactate dehydrogenase family (LDH) catalyzes the final step of anaerobic glycolysis, the conversion of L-lactate and NAD to pyruvate and NADH. The LDH family consists of three members, LDH-A, LDH-B and LDH-C, all of which form tetramers consisting four subunits. However, each family member displays a specific tissue distribution pattern with LDH-A and LDH-B predominant in several tissues, specifically LDH-A in muscle and LDH-B in heart, while LDH-C expression is confined to the testis and sperm. LDHs function as powerful markers for germ cell tumors. The genes encoding human LDH-A and LDH-C map to chromosome 11, while the human LDH-B gene maps to chromosome 12. Deficiency in the LDH-A gene is linked to exertional myoglobinuria.

背景文献

1. Rocha CS et al. Melatonin alters the glycolytic profile of Sertoli cells: implications for male fertility. Mol Hum Reprod 20:1067-76 (2014).
2. Mallat Y et al. Proteome modulation in H9c2 cardiac cells by microRNAs miR-378 and miR-378. Mol Cell Proteomics 13:18-29 (2014).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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