产品详情
产品名称Vitronectin Rabbit mAb
克隆号12D1
纯化ProA affinity purified
应用WB, ICC/IF, IHC
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名Complement S Protein antibody
Epibolin antibody
S Protein antibody
S-protein antibody
Serum Spreading Factor antibody
Serum-spreading factor antibody
Somatomedin B antibody
Somatomedin-B antibody
V75 antibody
Vitronectin V10 subunit antibody
Vitronectin V65 subunit antibody
VN antibody
VNT antibody
VTN antibody
VTNC_HUMAN antibody
数据库入口号Swiss-Prot#:P04004
计算分子量75 kDa
配方Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Vitronectin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Vitronectin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Vitronectin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Vitronectin antibody. Counter stained with hematoxylin.
ICC staining Vitronectin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Vitronectin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Vitronectin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Vitronectin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of Vitronectin expression in human Serum membrane lysate.
背景
A431, A549, MCF-7, HepG2, mouse liver tissue, mouse skin tissue, human liver tissue, human liver cancer tissue.
背景文献
1. Kapinas K et al. Bone matrix osteonectin limits prostate cancer cell growth and survival. Matrix Biol 31:299-307 (2012).
2. Tamay AG et al. Evaluation of the effects of gonadotropin-releasing hormone antagonist (GnRH-ant) and agonist (GnRH-a) in the prevention of postoperative adhesion formation in a rat model with immunohistochemical analysis. Fertil Steril 96:1230-3 (2011).