产品详情
产品名称AKT1 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号ST48-09
纯化ProA affinity purified
应用WB, ICC/IF, IHC, IP, FC
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名AKT 1 antibody
AKT antibody
AKT1 antibody
AKT1_HUMAN antibody
MGC99656 antibody
PKB antibody
PKB-ALPHA antibody
PRKBA antibody
Protein Kinase B Alpha antibody
Protein kinase B antibody
Proto-oncogene c-Akt antibody
RAC Alpha antibody
RAC antibody
RAC-alpha serine/threonine-protein kinase antibody
RAC-PK-alpha antibody
数据库入口号Swiss-Prot#:P31749
计算分子量56 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of AKT1 on MCF-7 cell lysates using anti-AKT1 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AKT1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-AKT1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-AKT1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-AKT1 antibody. Counter stained with hematoxylin.
Flow cytometric analysis of Hela cells with AKT1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
背景
The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKBβor RacPK-β) and Akt 3 (also designated PKB? or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases.
背景文献
1. Wang, H. et al. 2016. CSL regulates AKT to mediate androgen independence in prostate cancer progression. Prostate. 76: 140-50.
2. Wang, Z. et al. 2016. Protein 41N acts as a potential tumor suppressor linking PP1 to JNK-c-Jun pathway regulation in NSCLC. Oncotarget. 7: 509-23.
如果您使用该产品48884发表了文章,请通知我们,让我们可以引用您的文献。
et al,CCDC65, a Gene Knockout that leads to Early Death of Mice, acts as a potentially Novel Tumor Suppressor in Lung Adenocarcinoma. In Int J Biol Sci
on 2022 Jun 27 by Ziyan Zhang, Ping Xu, et al..PMID:35844805,
, (2022),
PMID: 35844805